Assessment of an In Vitro Tick Feeding System for the Successful Feeding of Adult Rhipicephalus appendiculatus Ticks

This study assessed the efficiency of a new in vitro tick feeding system for adult Rhipicephalus appendiculatus and compared impact different blood anticoagulating factors on their process. A total 10 feeders were each seeded with 30 or 60 R. adults. Bovine was added into unit changed every 12 h 4 to days during which attachment engorgement assessed. The observed after 80.0% (48/60), 75.8% (182/240), 70.8% (170/240) lithium heparin, citrate phosphate dextrose, defibrinated blood, respectively, no significant difference (p > 0.05) between anticoagulants used. However, ticks fed heparinized reached repletion status. successfully used feed until repletion. could be facilitate studies tick-pathogen interactions, such as those involved East Coast fever disease.